Details of the primers used
| Primer | Sequence (5`→3`) | Regions targeted |
|---|---|---|
| VκFR3-1 | TTCAG(C/T)GGCAGTGG(A/G)TCTGG | VκI, III, IV, V, VI |
| VκFR3-2 | TTCAGTGGCAGTGGG(G/T)CAGG | VκII |
| Jκ1 | TTTGAT(A/T/C)TCCACCTTGGTCCC | JκI-IV |
| Jκ2 | TTTAATCTCCAGTCGTGTCCC | JκV |
| Vλ-FR3 | GA(T/C)GAGGCTGA(T/C)TATTACTG | VλI-VII |
| Jλ1 | AGGACGGTGACCTTGGTCCC | JλI |
| Jλ2 | AGGACGGTCAGCT(G/T)GGT(C/G)CC | JλII, III, VII |