Table 1

Biochemical profile of strain HKU3 by conventional biochemical tests and the Vitek system (BACIL) and API system (50CHB/20E)

Biochemical reactions/enzymesConventionalVitek BACILAPI 50CHB/20E
CatalaseWeak +
Cytochrome oxidase+
Nitrate reduction++
β Galactosidase++
Arginine dihydrolase
Lysine decarboxylase
Ornithine decarboxylase
Citrate utilisation
Malonate utilisatiion
MBM acetate utilisation
H2S
Urease
Tryptophan deaminase
Indole
Acetoin
Gelatinase
Esculin hydrolysis+++
Casein hydrolysis
Fermentation/oxidation/assimilation of
    Glycerol
    Erythritol
    D-Arabinose
    L-Arabinose
    Ribose
    D-Xylose
    L-Xylose
    Adonitol
    β Methylxyloside
    Galactose
    D-Glucose
    D-Fructose
    D-Mannose
    L-Sorbose
    Rhamnose
    Dulcitol
    Inositol
    Mannitol
    Sorbitol
    α Methyl-D-mannoside
    α Methyl-D-glucoside
    N-Acetyl glucosamine
    Amygdalin
    Arbutin
    Salicin
    Cellobiose
    Maltose
    Lactose
    Melibiose
    Saccharose
    Trehalose
    Inulin
    Melezitose
    D-Raffinose
    Amidon
    Glycogene
    Xylitol
    β Gentiobiose
    D-Turanose
    D-Lyxose
    D-Tagatose
    D-Fucose
    L-Fucose
    D-Arabitol
    L-Arabitol
    Gluconate
    2 cetogluconate
    5 cetogluconate
    Sucrose
    Palatinose
    Amylopectin
    Potassium thiocyanate
    7% NaCl
    Mandelic acid
    Oleandomycin
    Sodium acetate+
    Polyamidohygrostreptin
    Nalidixic acid
Reduction of tetrazolium red
Identification99% Bacillus sphaericus77.5% Brevibacillus brevis
22.5% Bacillus sphaericus