Primers and probes used for detection of human papilloavirus type 16 (HPV-16) DNA by the polymerase chain reaction
| Primer/Probe | Position | Product (bp) | |
|---|---|---|---|
| The primers and probes for the E6 regions of HPV-6, HPV-16, and HPV-18 were the same as those reported by McNicol et al.25 | |||
| HPV-16 E1 | |||
| Sense (16E1-a) | 5′-ACCATGGCTGATCCTGCAG-3′ | 862–880 | 2087 |
| Antisense (16E1-b) | 5′-TGATACAGCCAGTGTTGGCA-3′ | 2930–2947 | |
| HPV-16 E2 | |||
| Sense (16E2-a) | 5′-TGGAAACACATGCGCCTAGA-3′ | 2851–2870 | 471 |
| Sense (16E2-a‘) | 5′-ATGGAGACTCTTTGCCAACG-3′ | 2755–2774 | 566 |
| Antisense (16E2-b) | 5′-ACCCGCATGAACTTCCCATA-3′ | 3302–3321 | |
| HPV-16 E4 | |||
| Sense (16E4-a) | 5′-AACGAAGTATCCTCTCCTGAA-3′ | 3361–3381 | 201 |
| Antisense (16E4-b) | 5′-GACAGTGCTCCAATCCTCACT-3′ | 3542–3561 | |
| HPV-16 E5 | |||
| Sense (16E5-a) | 5′-CATCCACAACATTACTGGCG-3′ | 3868–3887 | 228 |
| Antisense (16E5-b) | 5′-CACATGCACGCTTTTTAATTAC-3′ | 4074–4095 | |
| HPV-16 E6 | |||
| Sense (16E6-a) | 5′-CACCAAAAGAGAACTGCAATG-3′ | 86–106 | 413 |
| Sense (16E6-a‘) | 5′-GATGGGAATCCATATGCTGTA-3′ | 269–289 | 240 |
| Antisense (16E6-b) | 5′-TCGACCGGTCCACCGACCCCT-3′ | 488–508 | |
| Probe | 5′-GCCACTGTGTCCTGAAGAAAAGC-3′ | 427–449 | |
| HPV-16 E7 | |||
| Sense (16E7-a) | 5′-CCAGAGACAACTGATCTCTAC-3′ | 610–630 | 171 |
| Sense (16E7-a‘) | 5′-ATGCATGGAGATACACCTACA-3′ | 562–582 | 119 |
| Antisense (16E7-b) | 5′-GTGTGTGCTTTGTACGCACAAC-3′ | 759–780 | |
| Probe | 5′-TAACCTTTTGTTGCAAGTGTGACTCTACGCTTCG-3′ | 725–758 | |
| HPV-16 L1 | |||
| Sense (16L1-a) | 5′-TGCTAGTGCTTATGCAGCAA-3′ | 6029–6048 | 151 |
| Sense (16L1-a‘) | 5′-GGAGGACTGGAATTTTGGTC-3′ | 6833–6852 | 321 |
| Antisense (16L1-b) | 5′-ATTTACTGCAACATTGGTAC-3′ | 6160–6179 | |
| Antisense (16L1-b‘) | 5′-TTACAGCTTACGTTTTTTGCGT-3′ | 7133–7154 | |
| HPV-16 L2 | |||
| Sense (16L2-a) | 5′-TGCAGAAACTGGAGGGCATT-3′ | 4756–4775 | 368 |
| Antisense (16L2-b) | 5′-CTAGAGGTTAATGCTGGCCT-3′ | 5105–5124 | |