Oligonucleotide primer sequences for PCR analysis of GAPDH and zFOC1 transcripts
| Transcript | Oligonucleotide primer (nucleotide position in the mRNA) | PCR product size |
|---|---|---|
| The accession number is AL834266 for zFOC1 and M17851 for GAPDH. | ||
| GAPDH, glyceraldehyde 3 phosphate dehydrogenase. | ||
| zFOC1 | ||
| Forward primer | dGAGGAAGTAGCAGTCGCTGG (bp 3941–3959) | 349 bp |
| Reverse primer | dTCAAAGATCCTTAATTTCCC (bp 4289–4269) | |
| GAPDH | ||
| Forward primer | dGAGTCAACGGATTTGGTCGT (bp 53–72) | 158 bp |
| Reverse primer | dGGTGCCATGGAATTTGCCAT (bp 210–191) | |