Regular Article
Extracellular Role of S100A4 Calcium-Binding Protein in the Periodontal Ligament

https://doi.org/10.1006/bbrc.1999.0214Get rights and content

Abstract

S100A4 is a member of the S100 calcium-binding protein family. S100A4 is expressed in several tissues; however, it is secreted by few cell types and its extracellular roles are unknown. In the present study we showed byin situhybridization that periodontal ligament (PDL) cells express the S100A4 mRNA. Immunolocalization of the S100A4 protein in cryosections of PDL and analyses of PDL cell culture medium revealed that PDL cells secrete the S100A4 protein bothin vivoandin vitro.Interestingly, addition of a recombinant mouse S100A4 protein to a bone marrow cell culture inhibited mineralized nodule formation in a concentration-dependent manner. This is the first report of an extracellular role for S100A4 as an inhibitor of mineralization. The PDL space is kept free of mineralization and S100A4 may be one of the factors responsible for such phenomenon.

References (30)

  • B.W. Schafer et al.

    Trends Biochem. Sci.

    (1996)
  • F.E. Gibbs et al.

    J. Biol. Chem.

    (1994)
  • M.S. Grigorian et al.

    Gene

    (1993)
  • K. Takenaga et al.

    Biochem. Biophys. Res. Commun.

    (1994)
  • R. Barraclough et al.

    Biochem. Biophys. Res. Commun.

    (1990)
  • Y. Watanabe et al.

    J. Biol. Chem.

    (1992)
  • S. Kasugai et al.

    Arch. Oral Biol.

    (1990)
  • K. Takeda et al.

    Biochem. Biophys. Res. Commun.

    (1994)
  • A.S. Polans et al.

    J. Biol. Chem.

    (1994)
  • B.R. Davies et al.

    Oncogene

    (1993)
  • K. Takenaga et al.

    Cell Struct. Funct.

    (1994)
  • F.E. Gibbs et al.

    J. Histochem. Cytochem.

    (1995)
  • J. Klingelhofer et al.

    Dev. Dyn.

    (1997)
  • P. Lekic et al.

    Anat. Rec.

    (1996)
  • Cited by (57)

    • Purple sweet potato anthocyanins elicit calcium overload-induced cell death by inhibiting the calcium-binding protein S100A4 in acute lymphoblastic leukemia

      2021, Food Bioscience
      Citation Excerpt :

      This speculation was further confirmed by the Western blot results, showing that S100A4 was significantly downregulated by PSPAs (Fig. 7). As the S100A4 level decreased, the Ca2+ level increased (Fig. 4C), consistent with the results of previous studies (Darya et al., 2006; Duarte et al., 1999; Kotnova et al., 2019). Thus, we assumed that PSPAs promoted intracellular Ca2+ accumulation via inhibiting S100A4.

    View all citing articles on Scopus
    1

    To whom correspondence should be addressed at Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Fax: +81-3-5803-0190. E-mail:[email protected].

    View full text