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Insulin-like growth factor (IGF)-I,-II and IGF-binding proteins in the cyst fluid of a patient with astrocytoma

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Abstract

Insulin-like growth factor (IGF)-I,-II and IGF-binding proteins (IGFBPs) were demonstrated in the cyst fluid of a patient with a hypothalamic astrocytoma. The astrocytoma cyst fluid was subjected to gel chromatography at low pH and the IGF-I and IGF-II levels were measured by specific radioimmunoassays. Immunoreactive IGF-I and IGF-II levels were 19 ng/ml and 78 ng/ml respectively. Several-fold higher IGF-II values were obtained when cyst fluid was not extracted or was extracted with acid ethanol before radioimmunoassay analysis. The immunoreactive IGFBP-1 concentration was 26 ng/ml. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequent Western ligand blotting with [125I]IGF-II revealed bands at ≈200, 34.5, 29.5, 24 and 21 kD as visualized by autoradiography. Binding studies demonstrated that these binding proteins bind specifically [125I]IGF-I and [125I]IGF-II. These observations suggest that IGFs as well as IGF-binding proteins are produced by astrocytoma cells and may act in a paracrine or autocrine fashion capable of modulating the growth of astrocytoma tumours.

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Author notes

  1. Walter Zumkeller

    Present address: Kinderklinik, Universitätskrankenhaus Eppendorf, Martinistrasse 52, W-2000, Hamburg, Germany

Authors and Affiliations

  1. Department of Endocrinology, Karolinska Hospital and Institute, S-10401, Stockholm, Sweden

    Walter Zumkeller & Maria Sääf

  2. Department of Neurosurgery, Karolinska Hospital and Institute, S-10401, Stockholm, Sweden

    Tiit Rähn

Authors
  1. Walter Zumkeller
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  2. Maria Sääf
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  3. Tiit Rähn
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Zumkeller, W., Sääf, M. & Rähn, T. Insulin-like growth factor (IGF)-I,-II and IGF-binding proteins in the cyst fluid of a patient with astrocytoma. Child's Nerv Syst 9, 100–103 (1993). https://doi.org/10.1007/BF00305317

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