Abstract
The EoL-1 and EoL-3, human eosinophilic leukemia cell lines, have been used as models for studying the maturation and the function of human eosinophils. We investigated the effects of interferon-γ (IFN-γ) on superoxide anion (O −2 ) production of these cell lines and interleukin-5 (IL-5) mRNA expression in the EoL-1. O −2 was measured by chemiluminescence of MCLA, one ofcypridina luciferin analogs. The O −2 production of fMLP-stimulated EoL-1 and EoL-3 was increased by the IFN-γ treatment. IL-5 mRNA expression was detected in the IFN-γ-treated EoL-1 by reverse transcription-polymerase chain reaction (RT-PCR). Further, we examined IFN-γ receptor 1 mRNA expression in these cell lines and peripheral blood eosinophils by means of northern blot hybridization. IFN-γ receptor 1 mRNA was detected in the EoL-3 and the IFN-γ-treated EoL-1. A weak expression of IFN-γ receptor 1 mRNA was detected in peripheral blood eosinophils isolated from a patient with eosinophilia. These results suggest that IFN-γ may act on eosinophils directly through its receptor.
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Ishizuka, T., Morita, K., Hisada, T. et al. The direct effect of interferon-γ on human eosinophilic leukemia cell lines: The induction of interleukin-5 mRNA and the presence of an interferon-γ receptor. Inflammation 20, 151–163 (1996). https://doi.org/10.1007/BF01487402
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DOI: https://doi.org/10.1007/BF01487402