Abstract
The nucleotide sequences of theBamHI-H fragment of the HPRS16 strain of Marek's disease virus (MDV) and of its attenuated derivative HPRS16/att have been determined. The results show that in addition to the tandem expansion of a 132 bp sequence from two copies in HPRS16 virus to eight copies in HPRS16/att, nucleotide substitutions, deletions, and insertions were also noted. Several potential open reading frames (ORFs) were identified. One of these (ORF 13) encoded a deduced protein, mol wt 32 kD, which is likely to be the serotype-1 specific phosphoprotein expressed in tumours (1) and mapped to anEcoRI fragment withinBamHI-H (2). Our results suggest that this ORF is unlikely to be the B antigen (3). ORF 4, which had some similarity to CD4 and immunoglobulin M heavy chain, was encoded by a transcript that originated within the first copy of the 132 bp repeat. ORF 21, which mapped entirely within UL, encoded a deduced protein at least 322 amino acids long that had some similarity to varicella zoster virus (VZV) alpha trans-inducing protein. None of these ORFs was altered significantly by attenuation, except ORF 4 and another small ORF (ORF 3), 5′ of the 132 bp repeats, which would probably fail to be transcribed because of truncation of an RNA transcript.
Similar content being viewed by others
References
Naito M., Nakajima K., Iwa N., Ono K., Yoshida I., Konobe T., Ikuta K., Ueda S., Kato S., and Hirai K., Avian Pathology15, 503–510, 1980.
Cui Z., Yan D., and Lee L.F., Virus Genes3, 309–322, 1990.
Sithole I., Coussens P.M., Lee L.F., and Velicer L.F. in Kato S., Horiuchi T., Mikami T., and Hirai K. (eds).Advances in Marek's Disease Research. Osaka, Japan, 1988, pp. 148–154.
Buckmaster A.E., Scott S.D., Sanderson M.J., Boursnell M.E.G., Ross L.J.N., and Binns M.M., J Gen Virol69, 2033–2042, 1988.
Ross L.J.N., Binns M.M., and Pastorek, J., J Gen Virol72, 949–954, 1991.
Fukuchi K., Sudo M., Tanaka A., and Nonoyama M., J Virol53, 994–997, 1985.
Hirai K., Ikuta K., and Kato S., Virology115, 385–389, 1981.
Ross L.J.N., Milne B, and Biggs P.M., J Gen Virol64, 2785–2790, 1983.
Maotani K.A., Kanamori A., Ikuta K., Ueda S., Kato S., and Hirai K., J Virol58, 657–659, 1986.
Hirai K., Kanamori A., Niikura M., Ikuta K., and Kato S., in Kato S., Horiuchi T., Mikami T., and Hirai K. (eds).Advances in Marek's Disease Research. Osaka, Japan, 1988, pp. 140–147.
Sugaya K., Bradley G., Nonoyama M., and Tanaka A., J Virol64, 5773–5782, 1990.
Bradley G., Hayashi M., Lancz G., Tanaka A., and Nonoyama M., J Virol63, 2534–2542, 1989.
Maray T., Malkinson M., and Becker Y., Virus Genes2, 49–68, 1988.
Schat K.A., Buckmaster A., and Ross L.J.N., Int J Cancer44, 101–109, 1989.
Churchill A.E. and Biggs P.M., Nature221, 744–747, 1969.
Lee Y.S., Tanaka A., and Nonoyama M., Gene19, 185–190, 1982.
Deininger P.L., Anal Biochem129, 16–223, 1983.
Sanger F., Niklen S., and Coulson A.R., Proc Natl Acad Sci USA74, 5463–5467, 1977.
Boursnell M.E.G., Brown T.D.K., Foulds I.J., Green P.F., Tomley F.M., and Binns M.M., J Gen Virol68, 57–77.
Hong G.F., Biosci Rep1, 243–252, 1981.
Staden R., Nucleic Acids Res10, 4731–4751, 1982.
Staden R., Nucleic Acids Res12, 521–538, 1984.
Lipman D.L. and Pearson W.R., Science227, 1435–1441, 1985.
Ross L.J.N., Delorbe W., Varmus H.E., Bishop M.J., Brahic M., and Haase A., J Gen Virol57, 285–296, 1981.
Farrell P.J., Deininger P.L., Bankier A., and Barrell B., Proc Natl Acad Sci USA80, 1565–1569, 1983.
Lee L.E., Kieff E.D., Bachenheimer S., Roizman B., Spear P.G., Burmester B.R., and Nazerian K., J Virol7, 289–294, 1971.
McGeogh D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., and Taylor P., J Gen Virol69, 1531–1574, 1988.
Horwitz B.H., Weinstat D.L., and DiMaio D., J Virol63, 4515–4519, 1989.
Kemp B.E. and Pearson R.B., Topics Biosci15, 342–346, 1990.
Kozak M., Cell44, 283–292, 1986.
Cantello J.L., Anderson A.S., Franchesconi A., and Morgan R.W., J Virol65, 1584–1588, 1991.
Binns M.M. and Ross L.J.N., Virus Res12, 371–382, 1989.
Coussens P.M. and Velicer L.F., J Virol62, 2372–2379, 1988.
Scott S.D., Ross L.J.N., and Binns M.M., J Gen Virol70, 3055–3065, 1989.
Ross L.J.N., Sanderson M., Scott S.D., Binns M.M., Doel T., and Milne B., J Gen Virol70, 1789–1804, 1989.
Birnstiel M.L., Busslinger M., and Strub K., Cell41, 349–359, 1985.
Becker Y., Hadar J., Tabor E., Ben-Hur T., Raibstein I., Rosen A., and Darai S., Virology149, 255–259, 1986.
Stevens J.G., Wagner E.K., Devi-Rao G.B., Cook M.L., and Feldman L., Science235, 1056–1059, 1987.
Clark S.J., Jefferies W.A., Barclay A.N., Gagnon J., and Williams A., Proc Natl Acad Sci USA84, 1649–1653, 1987.
Kawamura M., Hayashi M., Furuichi T., Nonoyama M., Isogai E., and Namioka S., J Gen Virol72, 1105–1111.
Nakajima K., Ikuta K., Naoto M., Ueda S., Kato S., and Hirai K., J Gen Virol68, 1379–1389, 1987.
Ross L.J.N. and Milne B., in Kato S., Horiuchi T., Mikami T., and Hirai K. (eds).Advances in Marek's Disease Research. Osaka, Japan, 1988, pp. 43–49.
Davison A.J. and Scott J.E., J Gen Virol67, 1759–1816, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ross, N., Binns, M.M., Sanderson, M. et al. Alterations in DNA sequence and RNA transcription of theBamHI-H fragment accompany attenuation of oncogenic Marek's disease herpesvirus. Virus Genes 7, 33–51 (1993). https://doi.org/10.1007/BF01702347
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01702347