Complete nucleotide sequence of the Marek's disease virus ICP4 gene

doi:10.1016/0042-6822(92)90589-H

Virology

Volume 189, Issue 2, August 1992, Pages 657-667

https://doi.org/10.1016/0042-6822(92)90589-H Get rights and content

Abstract

The Marek's disease virus (MDV) gene encoding a homologue to the ICP4 protein of herpes simplex virus has been mapped to BamHI fragment A based on the physical map of the MDV genome (Fukuchi et al., 1984). The gene lies completely within the inverted repeat flanking the unique short region of the genome. The complete nucleotide sequence of the MDV ICP4 gene has been determined. The coding region is 4245 nucleotides long and has an overall G + C content of 52%. The MDV ICP4 protein is predicted to have a structure similar to that of ICP4-like proteins of other herpesviruses in that it has five distinct regions, the second and fourth of which are highly conserved. In addition, the protein contains the characteristic run of serine residues located toward its amino terminus. The MDV ICP4 gene is expressed in MDV-infected chicken embryo fibroblasts.

References (55)

R.I. Courtney et al.
Isolation and characterization of a large molecular weight polypeptide of herpes simplex virus type 1
Virology
(1974)
F.J. Grundy et al.
DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene
Virology
(1989)
M. Kozak
Point mutations define a sequence flanking the AUG initiation codon that modulates translation by eukaryotic ribosomes
Cell
(1986)
P. Mavromara-Nazos et al.
Regulation of herpes simplex virus 1 genes: αgene sequence requirements for transient induction of indicator genes regulated by β or late (γ) promoters
Virology
(1986)
T. Paterson et al.
Mutational dissection of the HSV-1 immediate-early protein Vmw 175 involved in transcriptional transactivation and repression
Virology
(1988)
L. Pereira et al.
Regulation of herpesvirus macromolecular synthesis. V. Properties of αpolypeptides made in HSV-1 and HSV-2 infected cells
Virology
(1977)
J.A. Blaho et al.
ICP4, the major regulatory protein of herpes simplex virus, shares features common to GTPbinding proteins and is adenylated and guanylated
J. Virol.
(1991)
A.E. Buckmaster et al.
Gene sequence and mapping data from Marek's disease virus and herpesvirus of turkeys: Implications for herpesvirus classification
J. Gen. Virol.
(1988)
B.W. Calnek
Marek's disease—A model for herpesvirus oncology
CRC Crit. Rev. Microbiol.
(1986)
B.W. Calnek et al.
Feather follicle epithelium: A source of enveloped and infectious cell-free herpesvirus from Marek's disease
Avian Dis.
(1970)

B.W. Calnek et al.

Spontaneous and induced herpesvirus genome expression in Marek's disease tumor cell lines

Infect. Immun.

(1981)

J.L. Cantello et al.

Isolation of a Marek's disease virus (MDV) recombinant containing the IacZ gene of Escherichia coli stably inserted within the MDV US2 gene

J. Virol.

(1991)

A.K. Cheung

Fine mapping of the immediate-early gene of the Indiana-Funkhauser strain of pseudorabies virus

J. Virol.

(1988)

A.K. Cheung

DNA nucleotide sequence analysis of the immediate-early gene of pseudorabies virus

Nucleic Acids Res.

(1989)

A.K. Cheung

Detection of pseudorabies virus transcripts in trigeminal ganglia of latently infected swine

J. Virol.

(1989)

A.E. Churchill et al.

Agent of Marek's disease in tissue culture

Nature (London)

(1967)

K.D. Croen et al.

Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses

N.A. DeLuca et al.

Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4

J. Virol.

(1985)

N.A. DeLuca et al.

Activation of immediate-early, early, and late promoters by temperature-sensitive and wildtype forms of herpes simplex virus type 1 protein ICP4

Mol. Cell. Biol.

(1985)

N.A. DeLuca et al.

Activities of herpes simplex virus type 1 (HSV-1) ICP4 genes specifying nonsense peptides

Nucleic Acids Res.

(1987)

N.A. DeLuca et al.

Physical and functional domains of the herpes simplex virus transcriptional regulatory protein ICP4

J. Virol.

(1988)

R.A.F. Dixon et al.

Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175

J. Virol.

(1980)

S.W. Faber et al.

Association of the herpes simplex virus regulatory protein ICP4 with specific nucleotide sequences in DNA

Nucleic Acids Res.

(1986)

L.T. Feldman

The molecular biology of herpes simplex virus latency

K. Fukuchi et al.

Structure of Marek's disease virus DNA: Detailed restriction enzyme map

J. Virol.

(1984)

R.N. Harty et al.

Mapping the termini and intron of the spliced immediate-early transcript of equine herpesvirus 1

J. Virol.

(1989)

R.A. Honess et al.

Regulation of herpes virus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins

J. Virol.

(1974)

Cited by (59)

Marek's disease: An update on oncogenic mechanisms and control
2000, Research in Veterinary Science
Marek’s disease ( $MD$ ) is a common lymphoproliferative disease of poultry caused by a highly contagious and oncogenic herpesvirus. In spite of the widespread use of highly effective $MD$ vaccines, recently there have been worrying trends in the evolution of $MD$ virus pathotypes towards greater virulence. In the last few years, there has been significant progress in determining the molecular structure of $MD$ virus and several genes that map within the repeat regions of the virus, such as Bam HI-H family, $ICP$ 4, meq and pp38, which are potentially associated with the latency and transformation have been identified. The functions of some of these genes have provided insights into the mechanisms of $MD$ virus-induced oncogenesis. This review summarises some of these oncogenic mechanisms and the progress in the control of $MD$ .
A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: Differences in two populations of lymphoma cells
1999, Journal of Virological Methods
A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNγ gene. Each set of primers was present in the same reaction tube so that the 327 bp ICP4 product and the 420 bp IFNγ product were co-amplified. Two different fluorescent dyes were used to 5′-end label one PCR primer of each pair to distinguish the IFNγ and ICP4 products by colour. The IFNγ PCR product was used as an internal standard enabling comparisons of MDV-ICP4 products between different samples. Neither duplex PCR product was preferentially amplified and both reactions were in their exponential phases when stopped. The products could be distinguished by both size and colour. MD lymphoma cells were taken ex vivo and separated on the basis of expressing a novel host surface antigen recognised by the monoclonal antibody AV37. AV37⁺ lymphoma cells had greater MDV-loads than AV37⁻ lymphoma cells. The principles used here should be applicable to any cell phenotype and/or cell-associated DNA virus.
Novel BNIP1 variants and their interaction with BCL2 family members
1999, FEBS Letters
By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members.
T-cell transformation by Marek's disease virus
1999, Trends in Microbiology
A novel effective and safe vaccine for prevention of marek’s disease caused by infection with a very virulent plus (Vv+) marek’s disease virus
2021, Vaccines
Neoplastic diseases
2019, Diseases of Poultry

View all citing articles on Scopus

^☆: Published as Miscellaneous Paper 1418 of the Delaware Agricultural Experiment Station. Sequence data from this article have been deposited with the EMBUGenBank Data Libraries under Accession No. M75729.

View full text

Complete nucleotide sequence of the Marek's disease virus ICP4 gene☆

Abstract

Virology

Virology

Cell

Virology

Virology

Virology

ICP4, the major regulatory protein of herpes simplex virus, shares features common to GTPbinding proteins and is adenylated and guanylated

J. Virol.

Gene sequence and mapping data from Marek's disease virus and herpesvirus of turkeys: Implications for herpesvirus classification

J. Gen. Virol.

Marek's disease—A model for herpesvirus oncology

CRC Crit. Rev. Microbiol.

Feather follicle epithelium: A source of enveloped and infectious cell-free herpesvirus from Marek's disease

Avian Dis.

Spontaneous and induced herpesvirus genome expression in Marek's disease tumor cell lines

Infect. Immun.

Isolation of a Marek's disease virus (MDV) recombinant containing the IacZ gene of Escherichia coli stably inserted within the MDV US2 gene

J. Virol.

Fine mapping of the immediate-early gene of the Indiana-Funkhauser strain of pseudorabies virus

J. Virol.

DNA nucleotide sequence analysis of the immediate-early gene of pseudorabies virus

Nucleic Acids Res.

Detection of pseudorabies virus transcripts in trigeminal ganglia of latently infected swine

J. Virol.

Agent of Marek's disease in tissue culture

Nature (London)

Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses

Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4

J. Virol.

Activation of immediate-early, early, and late promoters by temperature-sensitive and wildtype forms of herpes simplex virus type 1 protein ICP4

Mol. Cell. Biol.

Activities of herpes simplex virus type 1 (HSV-1) ICP4 genes specifying nonsense peptides

Nucleic Acids Res.

Physical and functional domains of the herpes simplex virus transcriptional regulatory protein ICP4

J. Virol.

Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175

J. Virol.

Association of the herpes simplex virus regulatory protein ICP4 with specific nucleotide sequences in DNA

Nucleic Acids Res.

The molecular biology of herpes simplex virus latency

Structure of Marek's disease virus DNA: Detailed restriction enzyme map

J. Virol.

Mapping the termini and intron of the spliced immediate-early transcript of equine herpesvirus 1

J. Virol.

Regulation of herpes virus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins

J. Virol.