Original contributionOptimal primer selection for clonality assessment by polymerase chain reaction analysis: I. Low grade B-cell lymphoproliferative disorders of nonfollicular center cell type
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2009, Human PathologyCitation Excerpt :In addition, 3 of the 6 identified EBV-positive specimens underwent double-labeling immunohistochemical staining for EBER and either CD3 or CD20. Polymerase chain reaction (PCR) was performed with DNA extracted from formalin-fixed paraffin-embedded tissue from 3 of the 6 identified EBV-positive specimens for immunoglobulin heavy chain gene (IGH) rearrangement using a fluorescently labeled consensus primer specific for framework III of the IGH variable regions and a primer specific for the IGH joining region as previously described (Invitrogen, Carlsbad, CA) [14]. PCR was also performed on these 3 specimens for T-cell receptor γ gene rearrangement using consensus primers specific for the T-cell receptor gamma gene variable and joining regions (Invitrogen) [15].
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Dr Segal is a recipient of an American Cancer Society Clinical Oncology Career Development Award.