Cell
Volume 60, Issue 3, 9 February 1990, Pages 487-494
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Article
Cyclin is a component of maturation-promoting factor from Xenopus

https://doi.org/10.1016/0092-8674(90)90599-AGet rights and content

Abstract

Highly purified maturation-promoting factor (MPF) from Xenopus eggs contains both cyclin B1 and cyclin B2 as shown by Western blotting and immunoprecipitation using Xenopus anti-B-type cyclin antibodies. Immunoprecipitates with these antibodies display the histone H1 kinase activity characteristic of MPF, for which exogenously added B1 and B2 cyclins are both substrates. Protein kinase activity against cyclin oscillates in maturing oocytes and activated eggs with the same kinetics as p34cdc2 kinase activity. These data indicate that B-type cyclin is the other component of MPF besides p34cdc2.

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      Cyclin/CDK complexes activate cell cycle proteins essential for transition to the next cell cycle phase, while CDK inhibitors interfere with cyclin/CDK complex formation (34). The decision to enter mitosis primarily depends on CDK1 activity after the initiation of the G2/M transition, and CDK1 and cyclin B form a complex to become part of the M-phase promoting factor (35, 36). Importantly, CDK1 activity is negatively regulated by WEE1, which catalyzes inhibitory phosphorylation of tyrosine 15 on CDK1 (37).

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    Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143.

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