Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes

Abstract

At least two types of somatic recombination are necessary for the generation of a complete immunoglobulin γ2b gene from germ-line DNA sequences. The first type of recombination consists of the assembly of three separate DNA segments, each encoding a different part of the variable region. The second type of recombination replaces the exons coding for the constant region of the μ chain with those coding for the same region of the -γ2b chain. The DNA sequencing studies suggest that the two types of recombination operate by different mechanisms.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).

    Article  ADS  CAS  Google Scholar 

  2. Tonegawa, S., Hozumi, N., Matthyssens, G. & Schuller, R. Cold Spring. Harb. Symp. quant. Biol. 41, 877–889 (1976).

    Article  Google Scholar 

  3. Brack, C., Hirama, M., Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

    Article  CAS  Google Scholar 

  4. Tonegawa, S. et al. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1978).

    Google Scholar 

  5. Seidman, J. G. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3881–3885 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).

    Article  CAS  Google Scholar 

  7. Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Seidman, J. G., Max, E. E. & Leder, P. Nature 280, 370–375 (1979).

    Article  ADS  CAS  Google Scholar 

  9. Lenhard-Schuller, R., Hohn, B., Brack, C., Hirama, M. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 4709–4713 (1978).

    Article  ADS  Google Scholar 

  10. Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Gilmore-Herbert, M. & Wall, R. Proc. natn. Acad. Sci. U.S.A. 75, 342–345 (1978).

    Article  ADS  Google Scholar 

  12. Schibler, U., Marcu, K. B. & Perry, R. P. Cell 15, 1495–1509 (1978).

    Article  CAS  Google Scholar 

  13. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1976).

    Article  ADS  Google Scholar 

  14. Weigert, M., Gatmaitan, L., Loh, E., Schilling, J. & Hood, L. Nature 276, 785–790 (1978).

    Article  ADS  CAS  Google Scholar 

  15. Weigert, M. et al. Nature 283, 497–499 (1980).

    Article  ADS  CAS  Google Scholar 

  16. Pernis, B., Forni, L. & Amante, L. Ann. N.Y. Acad. Sci. 190, 420–431 (1971).

    Article  ADS  CAS  Google Scholar 

  17. Pernis, B., Forni, L. & Luzzati, A. L. Cold Spring Harb. Symp. quant. Biol. 41, 175–183 (1976).

    Article  Google Scholar 

  18. Cooper, M. D., Kearney, J. F., Lydyard, P. M., Grossi, C. E. & Lawton, A. R. Cold Spring Harb. Symp. quant. Biol. 41, 139–145 (1976).

    Article  Google Scholar 

  19. Maki, R., Traunecker, A., Sakano, H., Roeder, W. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 77 (in the press).

  20. Davis, M. M. et al. Nature 283, 733–739 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Kataoka, T., Kawakami, T., Takahashi, N. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 77, 919–923 (1980).

    Article  ADS  CAS  Google Scholar 

  22. Kabat, E. A., Wu, T. T. & Bilofsky, H. in Sequences of Immunoglobulin Chains (NIH, Bethesda, 1979).

    Google Scholar 

  23. Breathnack, R., Benoist, C., O'Hare, K., Cannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  Google Scholar 

  24. Rabbitts, T. H., Matthyssens, G. & Hamlyn, P. H. Nature 284, 238–243 (1980).

    Article  ADS  CAS  Google Scholar 

  25. Davis, M., Early, P., Calame, K., Livant, D. & Hood, L. in Eucaryotic Gene Regulation (eds Axel, R., Maniatis, T. & Fox, C. F.) 393–406 (ICN–UCLA Symp., Academic, New York, 1979).

    Book  Google Scholar 

  26. Cory, S. & Adams, J. M. Cell, 19, 37–51 (1980).

    Article  CAS  Google Scholar 

  27. Schilling, J., Clevinger, B., Davie, J. M. & Hood, L. Nature 283, 35–40 (1980).

    Article  ADS  CAS  Google Scholar 

  28. Wang, J. C. Proc. natn. Acad. Sci. U.S.A. 76, 200–203 (1979).

    Article  ADS  CAS  Google Scholar 

  29. Salsano, P., Froland, S. S., Natvig, J. B. & Michaelsen, T. E. Scand. J. Immun. 3, 841–846 (1974).

    Article  CAS  Google Scholar 

  30. Fu, S. M., Winchester, R. J. & Kunkel, H. G. J. Immun. 114, 250–261 (1975).

    CAS  Google Scholar 

  31. Burrows, P., LeJeune, M. & Kearney, J. F. Nature 280, 838–840 (1974).

    Article  ADS  Google Scholar 

  32. Rao, D. N., Rudikoff, S., Krutzsch, H. & Potter, M. Proc. natn. Acad. Sci. U.S.A. 76, 2890–2894 (1979).

    Article  ADS  CAS  Google Scholar 

  33. Early, P. et al. Cell 19, 981–992 (1980).

    Article  CAS  Google Scholar 

  34. Kehry, M., Sibley, C., Fuhrman, J., Schilling, J. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 76, 2932–2936 (1979).

    Article  ADS  CAS  Google Scholar 

  35. Vrana, M., Rukikoff, S. & Potter, M. Proc. natn. Acad. Sci. U.S.A. 75, 1957–1961 (1978).

    Article  ADS  CAS  Google Scholar 

  36. Francis, S. H., Leslie, R. G. Q., Hood, L. & Eisen, H. N. Proc. natn. Acad. Sci. U.S.A. 71, 1123–1127 (1974).

    Article  ADS  CAS  Google Scholar 

  37. Milstein, C., Adetugbo, K., Cowan, N. J. & Seeker, D. S. Prog. Immun. 2, 157–168 (1974).

    Google Scholar 

  38. Bourgeois, A., Fougereau, M. & Depreval, C. Eur. J. Biochem. 24, 446–455 (1972).

    Article  Google Scholar 

  39. Rudikoff, S. & Potter, M. Proc. natn. Acad. Sci. U.S.A. 73, 2109–2112 (1976).

    Article  ADS  CAS  Google Scholar 

  40. Rudikoff, S. & Potter, M. Biochemistry 13, 4033–4038 (1974).

    Article  CAS  Google Scholar 

  41. IUPAC–IUB Commission J. Biochem. 113, 1 (1969).

  42. Smith, H. O. & Birnstiel, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).

    Article  CAS  Google Scholar 

  43. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1978).

    Article  ADS  Google Scholar 

  44. Hill, R. L., Delaney, R., Fellows, R. E. Jr & Lebovitz, H. E. Proc. natn. Acad. Sci. U.S.A. 56, 1762–1769 (1966).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakano, H., Maki, R., Kurosawa, Y. et al. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature 286, 676–683 (1980). https://doi.org/10.1038/286676a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/286676a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing