Abstract
The high-affinity receptor for immunoglobulin E, Fcɛ RI, is found exclusively on mast cells and basophils. When multivalent aller-gens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. In rodents Fcɛ RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding α subunit, one β subunit and a dimer of disulphide-linked γ subunits1. Com-plementary DNA encoding the α and the β subunits has recently been isolated2–5, but expression of IgE-binding by transfected cells has not yet been achieved2–5. Here we report the cloning of cDNA for the γ subunit, and propose a model for the αβγ2 tetramer which accounts for many of the structural features of the receptor. The rodent receptor on the surface of COS 7 cells was expressed only when the cDNAs for all three subunits were cotransfected. Successful expression of human IgE receptors should now be possible, eventually to permit the detailed analysis of the human IgE-receptor interaction and assist the search for therapeutically effective inhibitors.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Metzger, H. et al. A. Rev. Immun. 4, 419–470 (1986).
Kinet, J.-P., Metzger, H., Hakimi, J. & Kochan, J. Biochemistry 26, 4605–4610 (1987).
Shimizu, A. et al. Proc. natn. Acad. Sci. U.S.A. 85, 1907–1911 (1988).
Kochan, J., Pettine, L. F., Hakimi, J., Kishi, K. & Kinet, J.-P. Nucleic Acids Res. 16, 3584 (1988).
Kinet, J.-P. et al Proc. natn. Acad. Sci. U.S.A. 85, 6483–6487 (1988).
Alcaraz, G., Kinet, J.-P., Liu, T.-Y. & Metzger, H. Biochemistry 26, 2569–2575 (1987).
Kulczycki, A. Jr, Isersky, C. & Metzger, H. J. exp. Med. 139, 600–616 (1974).
McPhaul, M. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 83, 8863–8867 (1986).
Minami, Y., Weissman, A. M., Samelson, L. E. & Klausner, R. D. Proc. natn. Acad. Sci. U.S.A. 84, 2688–2692 (1987).
Rivnay, B., Wank, S. A. & Metzger, H. Biochemistry 21, 6922–6927 (1982).
Quarto, R., Kinet, J.-P. & Metzger, H. Molec. Immun. 22, 1045–1502 (1985).
Engelman, D. M., Steitz, T. A. & Goldman, A. A. Rev. Biophys. biophys. Chem. 15, 321–353 (1986).
Herskowitz, I. & Marsh, L. Cell 50, 995–996 (1987).
Cockcroft, S. & Gomperts, B. D. Nature 314, 534–536 (1985).
Holowka, D. & Baird, B. J. biol. Chem. 259, 3720–3728 (1984).
Quarto, R. & Metzger, H. Molec. Immun. 23, 1215–1223 (1986).
von Heijne, G. Biochim. biophys. Acta 947, 307–333 (1988).
Rivera, J., Kinet, J.-P., Kim, J., Pucillo, C. & Metzger, H. Molec. Immun. 25, 647–661 (1988).
Kinet, J.-P., Perez-Montfort, R. & Metzger, H. Biochemistry 22, 5729–5732 (1983).
Helm, B. et al. Nature 331, 180–183 (1988).
von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).
Liu, F.-T., Zinnecker, M., Hamaoka, T. & Katz, D. Biochemistry 18, 690–697 (1979).
Davis, L. G., Dibner, M. D. & Battey, J. F. in Basic Methods in Molecular Biology (ed. Davis, L. G.) (Elsevier, New York, 1986).
Liu, F.-T. et al. J. Immun. 124, 2728–2736 (1980).
Rittenberg, M. D. & Pratt, K. L. Proc. Soc. exp. Biol. Med. 132, 575–581 (1969).
Hibbs, M. L., Classon, B. J., Walker, I. D., McKenzie, I. F. C. & Hogarth, P. M. J. Immun. 140, 544–550 (1988).
Dayhoff, M. O., Hunt, L. T. & Hurst-Calderone, S. in Atlas of Protein Sequence and Structure Suppl. 3 (ed. Dayhoff, M. O.) 363–373 (Natn. biomed. Res. Fnd. Washington DC, 1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blank, U., Ra, C., Miller, L. et al. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337, 187–189 (1989). https://doi.org/10.1038/337187a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/337187a0
This article is cited by
-
Mast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile
Arthritis Research & Therapy (2016)
-
Happy 25th birthday, Bet v 1!
World Allergy Organization Journal (2014)
-
Dual regulation of mast cell degranulation through IgE receptor-mediated modulation of M2-type pyruvate kinase
Archives of Pharmacal Research (2014)
-
Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells
Molecular and Cellular Biochemistry (2014)
-
Type II phosphatidylinositol 4-kinases interact with FcεRIγ subunit in RBL-2H3 cells
Molecular and Cellular Biochemistry (2014)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.