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Crystal structure of the tyrosine kinase domain of the human insulin receptor

Abstract

The X-ray crystal structure of the tyrosine kinase domain of the human insulin receptor has been determined by multiwavelength anomalous diffraction phasing and refined to 2.1 Å resolution. The structure reveals the determinants of substrate preference for tyrosine rather than serine or threonine and a novel autoinhibition mechanism whereby one of the tyrosines that is autophosphorylated in response to insulin, Tyr 1,162, is bound in the active site.

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Hubbard, S., Wei, L. & Hendrickson, W. Crystal structure of the tyrosine kinase domain of the human insulin receptor. Nature 372, 746–754 (1994). https://doi.org/10.1038/372746a0

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