Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • On the Market
  • Published:

Gene expression profiles of laser-captured adjacent neuronal subtypes

A Correction to this article was published on 01 March 1999

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Laser capture microdissection from Nissl-stained sections (10 μm in thickness) of adult rat large and small DRG neurons.
Figure 2: cDNA microarray expression patterns of small (S) and large (L) neurons.
Figure 3: Representative fields of radioisotopic in situ hybridization of rat DRG with selected cDNAs.

References

  1. Shalon, D. Gene expression microarrays: A new tool for genomic research. Pathol. Biol. 46, 107–109 (1998).

    CAS  PubMed  Google Scholar 

  2. Lockhart, D.J. et al. Genomics and DNA chips. Nucleic Acids Symp. Ser. 38, 11–12 ( 1998).

    CAS  Google Scholar 

  3. Schena, M. et al. Microarrays: Biotechnology's discovery platform for functional genomics. Trends Biotechnol. 16, 301– 306 (1998).

    Article  CAS  Google Scholar 

  4. DeRisi, J.L., Iyer, V.R. & Brown, P.O. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278, 680– 686 (1997).

    Article  CAS  Google Scholar 

  5. Cho, R.J. et al. A genome-wide transcriptional analysis of the mitotic cell cycle. Mol. Cell 2, 65–73 (1998).

    Article  CAS  Google Scholar 

  6. Schena, M. et al. Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA 93, 10614–10619 (1996).

    Article  CAS  Google Scholar 

  7. Heller, R.A. et al. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc. Natl. Acad. Sci. USA 94, 2150–2155 (1997).

    Article  CAS  Google Scholar 

  8. Welford, S.M. et al. Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization. Nucleic Acids Res. 26, 3059 –3065 (1998).

    Article  CAS  Google Scholar 

  9. Emmert-Buck, M.R. et al. Laser capture microdissection. Science 274, 998–1001 (1996).

    Article  CAS  Google Scholar 

  10. Van Gelder, R.N. et al. Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc. Natl. Acad. Sci. USA 87, 1663 –1667 (1990).

    Article  CAS  Google Scholar 

  11. Schena, M., Shalon, D., Davis, R.W. & Brown, P.O. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270, 467–470 ( 1995).

    Article  CAS  Google Scholar 

  12. Coggeshall, R.E. & Willis, W.D. in Sensory Mechanisms of the Spinal Cord (Plenum, New York, 1991).

  13. Dib-Hajj, S.D., Tyrrell, L., Black, J.A. & Waxman, S.G. NaN, a novel voltage-gated Na channel, is expressed preferentially in peripheral sensory neurons and down-regulated after axotomy. Proc. Natl. Acad. Sci. USA 95, 8963–8968 ( 1998).

    Article  CAS  Google Scholar 

  14. Goldstein, M.E., Grant, P., House, S.B., Henken, D.B. & Gainer, H. Developmental regulation of two distinct neuronal phenotypes in rat dorsal root ganglia. Neuroscience 71, 243–258 (1996).

    Article  CAS  Google Scholar 

  15. Naciff, J.M., Kaetzel, M.A., Behbehani, M.M. & Dedman, J.R. Differential expression of annexins I–VI in the rat dorsal root ganglia and spinal cord. J. Comp. Neurol. 368, 356 –370 (1996).

    Article  CAS  Google Scholar 

  16. Liu, N., Fukami, K., Yu, H. & Takenawa, T. A new phospholipase C delta-4 is induced at S-phase of the cell cycle and appears in the nucleus. J. Biol. Chem. 271, 355– 360 (1996).

    Article  CAS  Google Scholar 

  17. Oh, Y., Sashihara, S., Black, J.A. & Waxman, S.G. Na+ channel beta 1 subunit mRNA: Differential expression in rat spinal sensory neurons. Mol. Brain Res. 30, 357–361 (1995).

    Article  CAS  Google Scholar 

  18. Nicke, A. et al. P2X1 and P2X3 receptors form stable trimers: A novel structural motif of ligand-gated ion channels. EMBO J. 17, 3016–3028 (1998).

    Article  CAS  Google Scholar 

  19. Costigan, M. et al. Heat shock protein 27: Developmental regulation and expression after peripheral nerve injury. J. Neurosci. 18, 5891–5900 (1998).

    Article  CAS  Google Scholar 

  20. Parysek, L.M., Chisholm, R.L., Ley, C.A. & Goldman, R.D. A type III intermediate filament gene is expressed in mature neurons. Neuron 1, 395–401 ( 1988).

    Article  CAS  Google Scholar 

  21. Liang, P. & Pardee, A.B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971 ( 1992).

    Article  CAS  Google Scholar 

  22. Kim, S.H. & Chung, J.M. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain 50, 355–363 ( 1992).

    Article  CAS  Google Scholar 

  23. Simmons, D.M., Arriza, J.L. & Swanson, L.W. A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radiolabeled single-stranded RNA probes. J. Histotechnol. 12, 169– 181 (1989).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank K. Witmeyer, K. Flores, J. Chambers, A. Leung and V. Le for their technical assistance.

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luo, L., Salunga, R., Guo, H. et al. Gene expression profiles of laser-captured adjacent neuronal subtypes . Nat Med 5, 117–122 (1999). https://doi.org/10.1038/4806

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/4806

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing