Abstract
Programmed cell death (PCD) in Dictyostelium shows a pattern of ordered degeneration similar to that observed in higher eukaryotes but somewhat different from the most studied form of PCD, i.e. apoptosis. To contribute to a genetic definition of this process, Dictyostelium HMX44A cells have been subjected to insertional mutagenesis, followed by selection based on several rounds of differentiation/regrowth to recover only cells resistant to death. We describe here the approach used, a partial characterization of the first mutant thus obtained called C5 showing some dissociation of cell death signs, and, in this case where plasmid rescue was not possible, as a first step towards identification of the gene at play recovery of genomic flanking sequences via genomic recircularization and PCR. This work demonstrates the feasibility of an insertional mutagenesis approach to obtain death-resistant mutants in Dictyostelium.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Corresponding author
Additional information
Edited by G. Melino
Rights and permissions
About this article
Cite this article
Cornillon, S., Olie, R. & Golstein, P. An insertional mutagenesis approach to Dictyostelium cell death. Cell Death Differ 5, 416–425 (1998). https://doi.org/10.1038/sj.cdd.4400361
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.cdd.4400361
Keywords
This article is cited by
-
Autophagy and cell death in model organisms
Cell Death & Differentiation (2009)
-
An adhesion molecule in free‐livingDictyosteliumamoebae with integrin β features
EMBO reports (2006)
-
Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis?
Cell Death & Differentiation (2002)