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Association of RACK1 and PKCβ with the common β-chain of the IL-5/IL-3/GM-CSF receptor

Oncogene volume 18, pages 5126–5130 (1999)Cite this article

Abstract

Granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-3 (IL-3) and interleukin-5 (IL-5 belong to a family of cytokines that regulate proliferation, differentiation and function of haematopoietic cells. Their receptor consists of a ligand specific α-chain and a signal transducing β-chain (βc). While, the role of phosphotyrosine residues in the βc as mediators of downstream signalling cascades has been established, little is known about non-phosphotyrosine mediated events. To identify proteins interacting with βc, we screened a yeast two-hybrid library with the intracellular domain of βc. We found that RACK1, a molecule associating with activated PKC, PLCγ and Src kinases, associated with the membrane proximal region of βc in both yeast two-hybrid, immunoprecipitation and GST-pull-down assays. The association of RACK1 was constitutive, demonstrating no alteration upon cellular stimulation. Furthermore, upon stimulation of cells with IL-5 or PMA, a complex of βc and PKCβ was found. Together, these findings suggest a novel role for RACK1 as a possible adapter molecule associating with the intracellular domain of cytokine receptors.

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References

  • Al-Shami A, Bourgoin SG and Naccache PH. . 1997 Blood 89: 1035–1044.

  • Baxter GT, Miller D, Kuo KC, Wada G and Owicki J. . 1992 Biochemistry 31: 10950–10954.

  • van der Bruggen T, Kok PTM, Raaijmakers JAM, Verhoeven AJ, Kessels RGC, Lammers J-WJ and Koenderman L. . 1993 J. Leukocyte Biol. 53: 347–353.

  • Burton EA, Hunter S, Wu SC and Anderson SM. . 1997 J. Biol. Chem. 272: 16189–16195.

  • Caldenhoven E, van Dijk T, Raaijmakers JAM, Lammers JWJ, Koenderman L and de Groot RP. . 1995 J. Biol. Chem. 270: 25778–25784.

  • Chang BY, Conroy KB, Machleder EM and Cartwright CA. . 1998 Mol. Cell. Biol. 18: 3245–3256.

  • Chihara J, Plumas J, Gruart V, Tavernier J, Prin L, Capron A and Capron M. . 1990 J. Exp. Med. 172: 1347–1351.

  • Chin H, Nakamura N, Kamiyama R, Miyasaka N, Ihle JN and Miura O. . 1996 Blood 88: 4415–4425.

  • Cloud-Heflin BA, McMasters RA, Osborn MT and Chambers TC. . 1996 Eur. J. Biochem. 239: 796–804.

  • Eder M, Griffin JD and Ernst TJ. . 1993 EMBO J. 12: 1647–1656.

  • Fujisawa T, Abu Ghazaleh R, Kita H, Sanderson CJ and Gleich GJ. . 1990 J. Immunol. 144: 642–646.

  • Gale RE, Freeburm RW, Khwaja A, Chopra R and Linch DC. . 1998 Blood 91: 54–63.

  • de Groot RP, van Dijk TB, Caldenhoven E, Coffer PJ, Raaijmakers JAM, Lammers J-WJ and Koenderman L. . 1997 J. Biol. Chem. 272: 2319–2325.

  • de Groot RP, Coffer PJ and Koenderman L. . 1998 Cell. Signal. 10: 619–628.

  • Gubina E, Rinaudo MS, Szallasi Z, Blumberg PM and Mufson RA. . 1998 Blood 91: 823–829.

  • Lanfrancone L, Pelicci G, Brizzi MF, Aronica MG, Casciari C, Giuli S, Pegorado L, Pawson T and Pellici PG. . 1995 Oncogene 10: 907–917.

  • Li Y, Shen BF, Karanes C, Sensenbrenner L and Chen B. . 1995 J. Immunol. 155: 2165–2174.

  • Liliental J and Chang DD. . 1998 J. Biol. Chem. 273: 2379–2383.

  • Lopez AF, Sanderson CJ, Gamble JR, Campbell HD, Young IG and Valdas MA. . 1988 J. Exp. Med. 167: 219–224.

  • Malizewski CR, March CJ, Schoenborn MA, Gimpel S and Shen L. . 1990 J. Exp. Med. 172: 1665–1672.

  • Murakami M, Narazaki M, Hibi M, Yawata H, Yasukawa K, Hamaguchi M, Taga T and Kishimoto T. . 1991 Proc. Natl. Acad. Sci. USA 88: 11349–11353.

  • Murata Y, Takaki S, Migita M, Kikuchi Y, Tominaga A and Takatsu K. . 1992 J. Exp. Med. 175: 341–351.

  • Padanilam BJ and Hammerman MR. . 1997 Am. J. Physiol. 272: F160–F166.

  • Pascale A, Fortino I, Govoni S, Trabucci M, Wetsel WC and Battaini F. . 1996 J. NeuroChem. 67: 2471–2477.

  • Pierce A, Heyworth CM, Nicholls SE, Spooncer E, Dexter TM, Lord JM, Owen-Lynch PJ, Wark G and Whetton AD. . 1998 J. Cell. Biol. 140: 1511–1518.

  • Quelle FW, Sato N, Witthuhn BA, Inhorn RC, Eder M, Miyajima A, Griffin JD and Ihle JN. . 1994 Mol. Cell. Biol. 14: 4335–4341.

  • Rinaudo MS, Su K, Falk LA, Haldar S and Mufson RA. . 1995 Blood 86: 80–88.

  • Ron D, Chen CH, Caldwell J, Jamieson L, Orr E and Mochly-Rosen D. . 1994 Proc. Natl. Acad. Sci. USA 91: 839–843.

  • Ron D, Luo J and Mochly-Rosen D. . 1995a J. Biol. Chem. 270: 24180–24187.

  • Ron D and Mochly-Rosen D. . 1995b Proc. Natl. Acad. Sci. USA 92: 492–496.

  • Rossi F, McNagny KM, Smith G, Frampton J and Graf T. . 1996 EMBO J. 15: 1894–1901.

  • Sanderson CJ, Warren DJ and Strath M. . 1985 J. Exp. Med. 162: 60–74.

  • Spaargaren M and Bischoff JR. . 1994 Proc. Natl. Acad. Sci. USA 91: 12609–12613.

  • Tavernier J, Devos R, Cornelis S, Tuypens T, van der Heyden J, Fiers W and Plaetinck G. . 1991 Cell 66: 1175–1184.

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  1. Marcel Spaargaren

    Present address: Department of Pathology, Academic Medical Center, University of Amsterdam, Meibergerdreef 9, Amsterdam, 1105 AZ, The Netherlands

Authors and Affiliations

  1. Department of Pulmonary Diseases, University Hospital Utrecht, Heidelberglaan 100, PO Box 85500, Utrecht, 3508 GA, The Netherlands

    Niels Geijsen, Jan AM Raaijmakers, Jan-Willem J Lammers, Leo Koenderman & Paul J Coffer

  2. Laboratory for Physiological Chemistry, Utrecht University, Universiteitsweg 100, Utrecht, 3584 CG, The Netherlands

    Marcel Spaargaren

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Geijsen, N., Spaargaren, M., Raaijmakers, J. et al. Association of RACK1 and PKCβ with the common β-chain of the IL-5/IL-3/GM-CSF receptor. Oncogene 18, 5126–5130 (1999). https://doi.org/10.1038/sj.onc.1202896

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