Abstract
The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Avian Myeloblastosis Virus / enzymology
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Avian Myeloblastosis Virus / genetics
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Base Sequence
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Bone Marrow Cells
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Cells, Cultured
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Chickens
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Cloning, Molecular
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Deoxyribonuclease I
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Escherichia coli / genetics
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Gene Expression Regulation, Neoplastic*
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Gene Library
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Genes, Regulator*
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Molecular Sequence Data
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Oncogene Proteins v-myb
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Oncogenes*
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Promoter Regions, Genetic
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Protein Binding
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Protein-Tyrosine Kinases / metabolism
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RNA, Messenger / genetics
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Retroviridae Proteins, Oncogenic / genetics
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Retroviridae Proteins, Oncogenic / metabolism*
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Transcriptional Activation
Substances
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Oncogene Proteins v-myb
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RNA, Messenger
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Retroviridae Proteins, Oncogenic
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Protein-Tyrosine Kinases
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Deoxyribonuclease I
Associated data
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GENBANK/M29448
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GENBANK/M29449