Abstract
To identify receptor tyrosine kinases (RTKs) critical to early hematopoiesis, we performed polymerase chain reaction-based cloning from yolk sac and highly enriched bone marrow hematopoietic stem cells (HSCs). Characterization of two novel genes of their full-length cDNA sequences revealed that they were mouse homologues of the endothelial cell RTK genes, TIE and TEK. They shared a unique structural property of coexistent immunoglobulin-like domain, epidermal growth factor-like repeats, and fibronectin type III repeats in their extracellular domains. Both genes were expressed in a similar fashion in adult tissues and primitive hematopoietic cells, predominantly in the bone marrow HSCs.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Bone Marrow / metabolism
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Cell Line
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Cloning, Molecular / methods
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Conserved Sequence
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Epidermal Growth Factor / genetics
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Fetus
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Gene Library
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Hematopoietic Stem Cells / metabolism*
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Humans
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Leukemia, Experimental
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Liver / metabolism
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Oligonucleotides, Antisense
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Protein Biosynthesis
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Protein-Tyrosine Kinases / biosynthesis
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Protein-Tyrosine Kinases / genetics*
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Proteins / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Cell Surface / biosynthesis
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Receptors, Cell Surface / genetics*
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Receptors, TIE
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Sequence Homology, Amino Acid
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Yolk Sac / metabolism
Substances
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Oligodeoxyribonucleotides
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Oligonucleotides, Antisense
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Proteins
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RNA, Messenger
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Receptors, Cell Surface
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Epidermal Growth Factor
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Protein-Tyrosine Kinases
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Receptors, TIE
Associated data
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GENBANK/L14790
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GENBANK/L14791
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GENBANK/L14792
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GENBANK/L14793
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GENBANK/L14794
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GENBANK/L14795
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GENBANK/L14796
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GENBANK/L14797
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GENBANK/L14798
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GENBANK/X73960