The direct effect of interferon-gamma on human eosinophilic leukemia cell lines: the induction of interleukin-5 mRNA and the presence of an interferon-gamma receptor

Inflammation. 1996 Apr;20(2):151-63. doi: 10.1007/BF01487402.

Abstract

The EoL-1 and EoL-3, human eosinophilic leukemia cell lines, have been used as models for studying the maturation and the function of human eosinophils. We investigated the effects of interferon-gamma (IFN-gamma) on superoxide anion (O2-) production of these cell lines and interleukin-5 (IL-5) mRNA expression in the EoL-1. O2- was measured by chemiluminescence of MCLA, one of cypridina luciferin analogs. The O2- production of fMLP-stimulated EoL-1 and EoL-3 was increased by the IFN-gamma treatment. IL-5 mRNA expression was detected in the IFN-gamma-treated EoL-1 by reverse transcription-polymerase chain reaction (RT-PCR). Further, we examined IFN-gamma receptor 1 mRNA expression in these cell lines and peripheral blood eosinophils by means of northern blot hybridization. IFN-gamma receptor 1 mRNA was detected in the EoL-3 and the IFN-gamma-treated EoL-1. A weak expression of IFN-gamma receptor 1 mRNA was detected in peripheral blood eosinophils isolated from a patient with eosinophilia. These results suggest that IFN-gamma may act on eosinophils directly through its receptor.

MeSH terms

  • Antigens, CD / biosynthesis*
  • Antigens, CD / genetics
  • Base Sequence
  • Eosinophils / drug effects*
  • Eosinophils / metabolism
  • Gene Expression Regulation, Leukemic / drug effects*
  • Humans
  • Imidazoles / pharmacology
  • Interferon gamma Receptor
  • Interferon-gamma / pharmacology*
  • Interleukin-5 / biosynthesis*
  • Interleukin-5 / genetics
  • Leukemia, Eosinophilic, Acute / pathology*
  • Luminescent Measurements
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Neoplastic Stem Cells / drug effects*
  • Neoplastic Stem Cells / metabolism
  • Pyrazines / pharmacology
  • RNA, Messenger / biosynthesis
  • Receptors, Interferon / biosynthesis*
  • Receptors, Interferon / genetics
  • Superoxides / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured

Substances

  • Antigens, CD
  • Imidazoles
  • Interleukin-5
  • Neoplasm Proteins
  • Pyrazines
  • RNA, Messenger
  • Receptors, Interferon
  • Superoxides
  • 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one
  • N-Formylmethionine Leucyl-Phenylalanine
  • Interferon-gamma
  • Tetradecanoylphorbol Acetate