Abstract
A model of spinal trauma was developed where spinal neurones of adult mice were exposed to the excitotoxic glutamate analogue beta-N-oxylamino-L-alanine (L-BOAA). After 24 h, the injured neurones received a single dose of [125I]-LIF at the same site of the spinal cord, and 2 h later, tissues were removed to assess the distribution of leukaemia inhibitory factor (LIF). There was a significant increase in LIF binding to the injured region of the spinal cord over saline controls, and this corresponded with a significant increase in LIF mRNA expression in the same region of the cord. There was a change in the expression of ciliary neurotrophic factor, but the expression of cardiotrophin-1 (CT-1) and the common receptor subunit LIF receptor beta (LIFRbeta) did not change after neurotoxin treatment. The results add to the evidence that LIF plays a significant role in the response of adult neuronal tissue to injury.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids, Diamino*
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Animals
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Ciliary Neurotrophic Factor
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Cytokines / biosynthesis
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Growth Inhibitors / biosynthesis*
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Growth Inhibitors / metabolism
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Interleukin-6*
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lymphokines / biosynthesis*
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Lymphokines / metabolism
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Mice
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Mice, Inbred C57BL
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Motor Neuron Disease / chemically induced
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Motor Neuron Disease / metabolism*
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Motor Neuron Disease / pathology
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Motor Neurons / metabolism
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Nerve Tissue Proteins / biosynthesis
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Neurotoxins / toxicity
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RNA, Messenger / biosynthesis
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Receptors, Cytokine / biosynthesis
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Receptors, Cytokine / metabolism
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Receptors, OSM-LIF
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Spinal Cord / metabolism
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Spinal Cord / pathology
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Spinal Diseases / chemically induced
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Spinal Diseases / metabolism*
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Spinal Diseases / pathology
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Up-Regulation
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beta-Alanine / analogs & derivatives
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beta-Alanine / toxicity
Substances
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Amino Acids, Diamino
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Ciliary Neurotrophic Factor
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Cytokines
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Growth Inhibitors
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Interleukin-6
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lif protein, mouse
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Lifr protein, mouse
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Lymphokines
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Nerve Tissue Proteins
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Neurotoxins
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RNA, Messenger
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Receptors, Cytokine
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Receptors, OSM-LIF
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beta-Alanine
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oxalyldiaminopropionic acid
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cardiotrophin 1