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A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification
  1. Kevin Morgan,
  2. Letty Lam,
  3. Noor Kalsheker
  1. Division of Clinical Chemistry, Department of Clinical Laboratory Sciences, Queen's Medical Centre, Nottingham NG7 2UH

    Abstract

    DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the α-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years.

    • PCR
    • DNA extraction
    • paraffin wax sections

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